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Cm. ” Includes bibliographical references and index. ISBN 978-1-118-36936-4 (cloth) – ISBN 978-1-118-84362-8 (ePDF) – ISBN 978-1-118-84343-7 (ePub) – ISBN 978-1-118-84342-0 (eMobi) – ISBN 978-1-118-84349-9 (oBK) I. , editor of compilation. II. , editor of compilation. III. Society for Biomaterials. [DNLM: 1. Biocompatible Materials. 2. Biomedical Engineering. 28–dc23 2013049566 Contributors Marta Alves da Silva, 3B's Research Group – Biomaterials, Biodegradables, and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, Portugal; ICVS/3B's – PT Government Associate Laboratory, Braga/Guimarães, Portugal Michael Ansorge, Universität Leipzig, Institute of Biochemistry, Leipzig, Germany Ezgi Antmen, BIOMATEN, METU Center of Excellence in Biomaterials and Tissue Engineering, Department of Biotechnology, Ankara, Turkey Anthony Atala, Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Winston-Salem, NC, USA Stephen F.
In this study, the carbonated hydroxyapatite surface accelerated differentiation and mineralization of the osteoprogenitor cells compared to the TCPS control as evidenced by more GFP expression at an earlier time point. 7 Fluorescence microscopy images of the calvarial cells from the transgenic mice on TCPS and cHA at 21 days with DAPI staining to show all cells (A,D), osteoblasts revealed by GFP expression (B,E), and XO staining for deposited mineral (C,F). Scale bar = 100 μm. 8 Differences in morphology revealed by the fluorescence microscopy images allow distinction between the cHA substrate (A) without cells and (B) with cell-deposited mineral.
6Col/GFP associated with preosteoblasts just prior to mineralization, and nontoxic xylenol orange (XO) staining of mineral taken from the same area in the cell culture plate at multiple time points. The cell reporter technology allows continuous monitoring of cell differentiation without requiring the use of dyes or antibody staining that require cell culture termination. 5 Scanning electron microscopy images of a carbonated hydroxyapatite coating at low and high magnification showing nanoscale, plate-like morphology.
