By Richard Pflanzer
Experimental and utilized body structure Laboratory guide, 8th version, is a accomplished, stand-alone laboratory guide for the one-semester body structure path taught on the undergraduate point. it may accompany any body structure textbook out there, and reinforces these ideas which are basic to all classes on body structure. The strengths of this lab handbook are its emphasis on hands-on experiments, a realistic stability of history details, and transparent procedural directions.
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Additional resources for Experimental and Applied Physiology Laboratory Manual
Condenser c. 7 Compound Light Microscopy 37 Pflanzer: Experimental and Applied Physiology Laboratory Manual, Seventh Edition 3. Compound Light Microscopy Text 7. Why should only lens paper be used to clean the ocular and the objectives? 8. Approximately how long has the light microscope been in use as a scientific tool? 8 © The McGraw−Hill Companies, 2003 Pflanzer: Experimental and Applied Physiology Laboratory Manual, Seventh Edition 4. Physical Processes of Biological Importance CHAPTER © The McGraw−Hill Companies, 2003 Text 4 Physical Processes of Biological Importance ■ INTRODUCTION An average 70-kilogram adult human consists of approximately 100 trillion cells organized into tissues, organs, and systems.
2. To become acquainted with the function of the major parts of the microscope. 3. To learn to use the compound light microscope in a competent and responsible manner. 4. To acquire an appreciation for the value of the microscope as an important tool of the scientist, educator, and student. Materials compound light microscope lens paper clean microscope slides clean coverslips toothpick dilute methylene blue prepared slide of microfilm prepared slide of human blood immersion oil xylene ■ EXPERIMENTAL METHODS Care of the Microscope Microscopes are costly and sensitive instruments that must be handled with care at all times.
Place one of the dishes containing congo red and one of the dishes containing methylene blue in the refrigerator. Record the temperature in the refrigerator. Dilute the congo red and the methylene blue solutions to 50% strength and fill the wells in the remaining two dishes. Place the four dishes (2 × 100% + 2 × 50%) on the laboratory bench and record the room temperature. Allow the dishes to remain undisturbed for at least 24 hours. During the next laboratory period, or at a time designated by the laboratory instructor, use a metric rule to measure the distance from the edge of the well to the diffusion edge in each of the four dishes.