By Stanley B. Kater, Charles Nicholson
The function of this e-book is to supply easy reference fabric to facilitate the additional improvement and alertness of the intracellular staining innovations which originated with the advent of Procion dye as a neurocellular stain. We had sev eral particular ambitions in compiling those chapters. First, we famous that either the strategies and result of intracellular staining were derived from various arrangements and released in various journals. for that reason, we attempted to make this knowledge extra on hand by means of incorporating a number of stories by means of unique authors. this could give you the reader with an information of the types of neurobiological prob lems for which intracellular staining has already been hired. A moment target used to be to facilitate extension of those how to a greater variety of difficulties. To this finish there are chapters facing Procion dye as a marker for ultrastructural investi gations, using cobalt as a staining compound and the implementation of computing device thoughts for quantitative reviews of neuronal relationships. As heritage for extra extension of those equipment this quantity includes fabric at the historical past of the invention of Procion dyes as intracellular stains, the chemistry of those dyes, and basics of fluorescence microscopy. Our ultimate particular aim was once to provide information of technical approaches that weren't available within the literature.
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Additional info for Intracellular Staining in Neurobiology
And K. Ikeda: Interneurons commanding swimmeret movements in the crayfish Procambarus clarki (Girard). Compo Biochem. Physio!. 12, 509-525 (1964). Discussion DR. ROBERT SHLAER (Northwestern University): Does the injected dye only fill cells by diffusion or by an active process? If not by an active process, perhaps Procion can be bonded to something such as a small protein to make it fill cells actively. I would like to comment that in looking at the fine dendritic detail in the cerebral cortex, much of it seems to be the result of processes going around other structures-blood vessels, cell bodies, and so on.
And R. ]. Walker: Marking individual nerve cells through electrophoresis of ferrocyanide from a microelectrode. Stain Techno!. 37, 217-219 (1962). Kravitz, E. , A. O. W. Stretton, ]. Alvarez, and E. ]. Furshpan: Determination of neuronal 40 A. O. W. Stretton and E. A. Kravitz geometry using an intracellular dye injection technique. Fedn Proe. Fedn Am. Socs expo Bio!. 27,749 (1968). , E. A. Kravitz, and D. D. Potter: The 'Y-aminobutyric acid (GABA) content of cell bodies of excitatory and inhibitory neurons of the lobster.
Recently, Maraglas has also been used as an embedding agent (Erlandson, 1964). The optical clarity of Maraglas makes it particularly effective for viewing whole mounts and blocks cut easily with a steel knife. Photographic enlargements of phase-contrast images (on semitransparent paper) of the serial sections were made. A Zeiss projection microscope was used for this purpose and enlarged images A. O. W. Stretton and E. A. ;::; a. ;::; '" a:: Qi 250 300 350 400 450 500 550 600 Wavelength Fig. 3.