By Avraham Rasooly, Keith E. Herold
This quantity represents a useful number of cellular well-being (mHealth) rising applied sciences. Chapters concentrate on 3 major components of mHealth: applied sciences for in vitro and environmental trying out, mHealth applied sciences for physiological and anatomical measurements and mHealth applied sciences for imaging. This ebook is designed to make mHealth extra available and comprehensible to engineers, doctors, molecular biologists, chemical, and actual technological know-how researchers constructing mHealth applied sciences.
Written within the hugely profitable Methods in Molecular Biology sequence layout, chapters comprise introductions to their respective issues, technical information regarding fabrics and reagents, step by step, effortlessly reproducible laboratory protocols, and key tips about troubleshooting and heading off recognized pitfalls.
Authoritative and functional, Mobile HealthTechnologies : tools in Molecular Biology goals to enhance entry to clinical approaches together with early detection, diagnostics and remedy in the course of the improvement of recent moveable and available units, and that it will result in greater well-being technologies.
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Extra info for Mobile Health Technologies: Methods and Protocols
Fig. 6 An example of real time detection. Fluorescence emission intensity as a function of time when the sample contains 0 (negative control), 102, 103, and 104 HIV copies/ml. (Inset ) The threshold time as a function of HIV concentration 6. Repeat the wash step above. 7. Dry the membrane to remove residual ethanol by aspirating or blowing dry air through inlet port with a 10-ml syringe. 8. Inject amplification mix (with correct proportion of enzymes, primers, nucleotides, salts, and dye) into the reactor as proportioned for volume of amplification chamber.
The health provider can contact a pharmacy and order prescriptions, provide additional guidance remotely, and encourage/discourage an office visit as appropriate. This chapter describes the development of a microfluidic cassette for nucleic acid based pathogen assays (with specific application to HIV viral load testing), and which is compatible with cell phone-based end-point detection of fluorescence signals generated in real-time enzymatic amplification of a pathogen target. The approach and methods reported here are sufficiently generic and can be readily adapted to other POC diagnostics applications.
1 Chip Design and Fabrication The microfluidic chips (Fig. 1) are fabricated in clear (transparent) plastic materials as bonded laminates. We have tested three different thermoplastic polymers: polycarbonate (PC), acrylic (PMMA), and cyclic olefin copolymer (COC) (Table 1). The chips have been rapidly prototyped using computer-aided design (CAD) and computer-aided manufacturing software (SolidWorks™, MasterCAM™, or AutoCAD™). , Universal Laser Systems, Scottsdale, AZ, 30–50 W power) to define the microfluidic circuit(s) and other features (Fig.