By Maureen V. Chadwick
Mycobacteria covers a few points of mycobacterial identity. The ebook begins by way of describing a old assessment of tuberculosis, together with subject matters approximately looking for its reasons and remedy and concerning the use of chemotherapy for treating tuberculosis. The textual content describes the several species of Mycobacteriaceae, together with Mycobacterium tuberculosis, M. africanum, M. bovis, M. microti, and M. ulcerans. security precautions in pathology laboratories; the coaching of fine tradition media and sensitivity try out media; and the remedy of specimens also are thought of. The ebook extra tackles sensitivity trying out of Mycobacteria utilizing the resistance ratio strategy, the proportional strategy and absolutely the focus strategy, and techniques of deciding upon mycobacteria. The textual content additionally describes assay tools for anti-tuberculosis medicinal drugs. Pathologists, scientific technologists, and people operating in pathology laboratories will locate the publication worthwhile.
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Extra resources for Mycobacteria. Institute of Medical Laboratory Sciences Monographs
Centrifuge the specimen and from the deposit inoculate two Lowenstein-Jensen slopes and prepare a smear for acid-fast bacilli. 2. Add 4% NaOH to the rest of the deposit and treat as for sputum. Two sets of inoculations are carried out on this specimen to enhance the chances of isolation. The untreated specimen may contain only a few tubercle bacilli which might be killed by the sodium hydroxide. The sample is then treated to kill any other bacteria which might be present and which would contaminate the Lowenstein-Jensen medium.
M. tuberculosis is norm ally sensitive to all the above antituberculosis drugs unless drug resistance has developed; with the atypical my cobacteria many are naturally resistant to the drugs. All atypical my co bacteria with the exception of M. xenopi are highly resistant to INH. M. xenopi has an MIC of about 0-25 Mg/ml, making it relatively sensitive to INH. Runyon Group III mycobacteria, again with the exception of M. xenopi, are highly resistant to most antituberculosis drugs except for ethionamide and cycloserine.
Resuspend deposit in sterile distilled water (or penicillin water, about 15-20 ml). 5. Centrifuge at 3000 r/min for 30 minutes. 6. Inoculate two Lowenstein-Jensen slopes as described in the Petroff method. N-AcetyUL-Cystine (NAC) This mucolytic agent has been used with sodium hydroxide in the treatment of sputum for the isolation of mycobacteria. In comparisons of 4% NaOH and 2% NaOH plus NAC, Kubica and his colleagues (1963, 1964) showed an increase of about THE TREATMENT OF SPECIMENS 47 30% in the number of positive cultures obtained using NAC.