A. Polliack's Normal, Transformed and Leukemic Leukocytes: A Scanning PDF

By A. Polliack

The scanning electron microscope (SEM) has been used with expanding frequency in recent times to check the outside mor­ phology of ordinary, remodeled and malignant leukocytes. because the unique studies on serious point-dried lymphocytes released in 1973, result of different stories utilizing better equipment were suggested giving upward push to a few controversy during this box and this can be mentioned within the textual content of the atlas. Advances in preparatory thoughts recorded prior to now three years have additionally contributed a lot to a greater figuring out of cellphone floor phenomena as visible less than the SEM. The textual content of the atlas strains the advancements during this box chronologically, summarizes the to be had literature and provides the present scenario within the mild of the newest reviews during this box. the pictures have been chosen to demonstrate the spectrum of floor morphology of the various telephone kinds acquired from common participants and sufferers with affliction states. expectantly, the atlas will function a advisor for destiny reports and for example of what SEM has to supply in offering information of floor architecture.

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Extra resources for Normal, Transformed and Leukemic Leukocytes: A Scanning Electron Microscopy Atlas

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The latter may be misidentified as lymphocytes in mixed populations of leukocytes; Wetzel and co-workers [100], using a positive method for identifying the same cells under the light microscope and the SEM, have pointed out this difficulty as a potential source of error. Examination of a single case of marked eosinophilia [72] confirmed this observation (Figs. 60, 61). Fortunately, eosinophils and basophils are present in small numbers in the peripheral blood and are infrequently encountered in enriched populations of lymphocytes separated by the Ficoll-Hypaque method.

Close-up photographs of granulocytes (from normal individuals and from others with granulocytosis), showing transverse ridge-like profiles and ruffles, which are sometimes polarized to one edge of the cell (x 71 00) 30 Granulocytes Fig. 58. Circulating granulocyte with prominent ridge-like profiles and peripheral ruffles (x 7100) Fig. 59. at ridge-like profiles and very well-developed basal and peripheral ruffles (x 14,400) Granulocytes 31 60 _ _'----' 61 Figs. 60 and 6l. Circulating eosinophilic leukocytes isolated from a patient with marked eosinophilia in which almost all cells in the peripheral blood were eosinophils (more than 95%).

The marker sometimes obscures visualization of the underlying surface detail and prevents accurate quantitation and precise localization of the receptors. In addition, the antibody can also adhere nonspecifically to many surfaces and dissociate from the marker making interpretation difficult in some cases. Furthermore, the marker may enter the cell, bind in layers or be removed during preparatory procedures and in critical pointdrying. 54 Murine Lymphocytes Labeled Using Tobacco Mosaic Virus (TMV) as a Label Fig.

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