By J. M. Widholm (auth.), Dr. Wolfgang G. W. Kurz (eds.)
At the tip of the preliminary assembly on basic and Secondary Metabolism of Plant cellphone Cultures at Schloss Rauischholzhausen, it used to be made up our minds to convene comparable occasions regularly halfway among the foreign Congress for Plant Tissue and telephone Cul ture. We felt it used to be essential to carry representatives of the study groups operating during this box jointly to evaluate development in addition to to debate destiny instructions. The Plant Biotechnology Institute of the nationwide learn Council of Canada in Saskatoon, Saskatchewan was once selected to be the location for the second one symposium held in 1988. The subject of this assembly used to be "The rules of fundamental and Secondary Metabol ism in Plant Cells" and judging through the contributions released during this quantity it was once a well timed and genuine one certainly. i want to thank my organizing committee and my learn workforce for his or her efforts, the nationwide learn Council of Canada for the monetary suggestions and the contributors for his or her contributions which all contributed to make this symposium successful. a distinct thanks to Mrs. L. -Blashill and R. Gallays for his or her tips in internet hosting this occasion. final yet no longer least i want to thank our writer, Springer-Verlag, Heidelberg for his or her deci sion to submit the lawsuits of this and destiny symposia.
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Additional resources for Primary and Secondary Metabolism of Plant Cell Cultures II
Example text
In cultured cells, the changes in activities of photochemical reactions did not closely follow the changes in ChI content. These results suggested that in green leaves, the ChI biosynthesis and degradation are almost coincident with the photosynthetic function, while in cultured cells, these parameters were not cooperative. The large cell volume of cultured cells could contribute to the low photosynthetic activities, especially on the high CO 2 compensation point. Less activities of RuBisCo and carbonic anhydrase and high respiration activity have been thought to be the main reason for the high CO 2 compensation point [9,12-14,20].
However, the second cell line derived from cultivar "Target" consistently displayed (over five subcultures) a greater dry weight yield on Miglyol than in its absence (Fig. 7). U sing iodine to stain for starch, microscope examination revealed that these cells were packed full of starch grains. The Miglyol added to the medium of this cell line became "waxy" in appearance and at the point of each subculture consisted partially of solid lumps rather than the usual liquid spherical globules. The observations could be explained as either a stress response of the cells to the lipophilic phase, or a utilization of a portion of the Miglyol via, for example, lipase degradation of the triglycerides liberating glycerol and free fatty acids.
Under photoautotrophic conditions the aromatic glucose esters are barely detectable in the cells and phenylalanine ammonia lyase shows very low enzyme activity. The induction of both PAL and the accumulation ofthe cinnamic acid esters can be titrated with sucrose. 5-1 % sucrose maximum expression has been found. This concentration 44 W. Hiisemann et al. 5% sucrose has repeatedly been found in our reversion experiments as the regulatory switching point between the photoautotrophic and heterotrophic mode of nutrition.
