By Dr. William J. Dickinson, Dr. David T. Sullivan (auth.)
There used to be a interval within the heritage of recent biology while proteins have been considered "gene items" in a slightly direct feel. An account in their visual appeal and disappearance during improvement and differentiation therefore appeared a suitable technique of describing "gene regulation". whilst RNA was once chanced on to be the instant manufactured from genetic job, the examine of proteins as gene items misplaced a few of its unique charm. certainly, the improvement of the strong approach to nucleic acid hybridization aroused the wish that an enormous array of particular messenger-RNA molecules synthesized in the course of cellphone differentiation will be separately assayed. The problems within the method of such formidable initiatives have been defined in quantity three of this sequence: Nucleic Acid Hybridization within the examine of telephone Differentiation (ed. H. URSPRUNG, 1972). Enzymes are in huge degree accountable for telephone functionality. essentially, their synthesis needs to be lower than genetic regulate. we're confident that the learn of enzyme habit in the course of improvement benefits a lot consciousness, fairly if the paintings is performed on a eukaryote that lends itself to genetic and developmental research. a powerful volume of genetic info is accessible at the insect Drosophila, and masses has been learnt approximately its improvement. the enormous chromosomes found in numerous tissues of this insect could good be priceless in a continuous research of the looks of particular enzymes and the transcription of the cognate genetic loci.
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Additional info for Gene-Enzyme Systems in Drosophila
Example text
The other class, usually referred to as the red pigments, are pteridines which are products of purine metabolism. The genetic aspects of the production of these pigments m Drosophila and other insects has been reviewed by ZIEGLER (1961). Analysis by Transplantation EPHRUSSI and BEADLE (1936) developed the eye disc transplantation technique in order to study the role of various genes and their interaction in controlling the development of the normal reddish brown color which is characteristic of the wildtype eye.
53). Useful deletions or other chromosomal rearrangements are detected by their effects on visible loci linked to the enzyme locus in question, and are then tested to see whether they overlap the enzyme locus as well. A deletion that overlaps (or extends into} an enzyme locus behaves like a null allele in tests with other alleles at the locus. An example is described on p. 75. Recently, STEWART and MERRIAM (1974) have shown that segmental aneuploids can be used to obtain cytogenetic localizations.
This new allele also causes the absence of the same isoaccepting form of tryosyl-tRNA, which is absent in su (sl' flies. TWARDZIK et al. (1971) concluded that since su (s) is recessive and affects the distribution of tRNA species and not the absolute amount of tRNA present, the wild-type allele of su (s) is a gene which functions to produce a factor involved in tRNA modification, and is not a structural gene for tyrosyltRNA. The relation between the absence of a particular tRNA and the restoration of tryptophan oxygenase accomplished by su (s) has been examined by recent experiments of jACOBSON (1971).
